Simple, Sensitive DNA and RNA Quantitation
- Easy, intuitive controls
- Ready to use with QuantiFluor® Dyes
- Recommended for next-generation sequencing
- Bundled package available for integrated instrumentation and assay
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Quantus™ Fluorometer and NGS Starter Package
Highly Sensitive, Affordable, Single-Tube Fluorometer
The Quantus™ Fluorometer is a compact, dual-channel fluorometer designed to provide highly sensitive fluorescent detection when quantifying nucleic acids. The Quantus™ Fluorometer is optimized with preprogrammed settings for Promega QuantiFluor® Dye Systems (QuantiFluor® dsDNA, RNA and ssDNA Systems) to quantitate nucleic acids and offers the flexibility to create customized methods and quantitation settings for other fluorescent dyes.
Features
- Easy, intuitive controls.
- Calculates concentration automatically.
- Store the last 50 readings and transfer data in real time.
- Recommended for NGS and compatible with Illumina (HiSeq/miSeq), Roche (454) and LifeTech (ion Torrent and ion Proton) Systems.
Two fluorescent channels:
- Red:Ex: 640nm shortpass (wavelengths up to 640nm); Em: 660–720nm
- Blue:Ex: 495nm shortpass (wavelengths up to 495nm); Em: 510–580nm
Easily customize sample names,view concentration results, raw values, sample volumes and a quality check.
Compact size at only 22.7 x 11.5 x 4.5cm
Large 3-inch liquid crystal display screen
Intuitive navigation
Performance
Detection Sensitivity
Fluorescence-based nucleic acid quantification methods are more sensitive than absorbance-based methods, allowing you to quantitate low-level DNA samples accurately.
Broad Dynamic Range
Complement your absorbance-based methods and quantify your target molecule over a wider range of concentrations.
Calibration and Sample Measurement
The calibration process eliminates the need to generate a standard curve for each set of unknown samples. Simply measure the fluorescence of your blank sample and the standard that represents the highest point on your standard curve. The Quantus™ Fluorometer saves the calibration data for future measurements.
No computer is necessary to use the Quantus™ Fluorometer, but after installing the free Quantus™ Software you can automatically transfer your quantitation results from the Quantus™ Fluorometer to a personal computer in real time. Export results easily into Microsoft® Excel.
Workflow
The high sensitivity makes the Quantus™ Fluorometer perfect when working with samples types such as formalin-fixed paraffin-embedded (FFPE) tissue that contain low levels of nucleic acid.
You can confidently quantify nucleic acid from FFPE tissues and proceed to your downstream assays, knowing that there is DNA or RNA present.
Successful and accurate quantification leads to higher success in downstream assays such as PCR, cloning, transfection and next-generation sequencing.
DNA Quantitation for NGS
Highly sensitive and easy-to-use DNA quantitation for your NGS applications all in one discounted bundle. QuantiFluor® dyes are optimized for high sensitivity, broad dynamic range and target specificity on the Quantus™ Fluorometer. The Quantus™ NGS Starter package is cost-effective and easy to incorporate into your laboratory.
- Add-and-read format makes measuring low concentrations of dsDNA simple—no dilutions, no extra tubes.
- Significantly increased sensitivity over absorbance at 260nm (NanoDrop® spectrophotometer) for those samples that are low in concentration.
- Expect high specificity to dsDNA with minimal binding to ssDNA, RNA, protein and interfering compounds.
- Successfully used in several NGS systems including Illumina (HiSeq/miSeq), Roche (454) and Thermo Fisher (Ion Torrent™ and Ion Proton™) systems.
Quantus™ NGS Starter Package (Cat.# E5150)
The Quantus™ NGS Starter Package includes the Quantus™ Fluorometer (Cat.# E6150), QuantiFluor® ONE dsDNA System (Cat.# E4870) and 0.5ml PCR Tubes (Cat.# E4941).
QuantiFluor® ONE dsDNA System
Provides a fluorescent double-stranded DNA-binding dye in an "add-and-read" format for both dye and standard, simplifying DNA quantitation and speeding up your workflow. It's as easy to use as NanoDrop® absorbance-based methods but much more sensitive for low-concentration samples.
Learn more
Available Separately
| Product | Catalog # |
|---|---|
| 0.5ml Thin-Walled PCR Tubes (50 tubes) | E4941 |
| 0.5ml Thin-Walled PCR Tubes (200 tubes) | E4942 |
Service and Support
The Quantus™ Fluorometer is backed by a one-year warranty.
Instrument Registration Contact Technical Services
Design Features
| Size | 22.7 × 11.5 × 4.5cm (L × W × H) |
| Weight | 400 grams |
| Linear Dynamic Range | Five orders of magnitude, assay-dependent |
| Detectors | Solid-state silicon sensor |
| Excitation Filters | Red: 640nm shortpass (wavelengths up to 640nm) Blue: 495nm shortpass (wavelengths up to 495nm) |
| Emission Filters | Red: 660–720nm Blue: 510–580nm |
| Calibration Type | Single-point (blank and standard) |
| Sample Tube | We recommend Axygen thin-walled PCR tubes (Axygen Cat.# PCR-05-C, available from Fisher or VWR) |
| Automatic Sleep Mode | After 1 hour of inactivity |
| Power Requirements | 5V, 0.2A Maximum |
| Operating System Requirements (for optional Quantus™ Software) | Windows® 10,Windows® 8, Windows® 7 (32- or 64-bit) .NET Framework 4.0 or later At least one USB port available |
Frequently Asked Questions
Quantus™ Fluorometer Frequently Asked Questions
What is the Quantus™ Fluorometer?
The Quantus™ Fluorometer is an easy-to-use and affordable single-tube fluorometer. The compact instrument offers intuitive navigation and a large 3-inch liquid crystal display screen. The fluorometer is preprogrammed with protocols for single-stranded DNA, double-stranded DNA and RNA quantitation using the QuantiFluor® Dyes but is also compatible with other fluorescent assays.
What are the excitation and emission wavelengths?
The Quantus™ Fluorometer is equipped with two fluorescence channels for nucleic acid and protein quantitation:
Blue fluorescence channel: Excitation 495nm shortpass (wavelengths up to 495nm), emission 510–580nm.
Red fluorescence channel: Excitation 640nm shortpass (wavelengths up to 640nm), emission 660–720nm.
How sensitive is the Quantus™ Fluorometer?
When used with the QuantiFluor® Dyes, the Quantus™ Fluorometer provides sensitivities that are up to 40,000 times higher than that of absorbance-based quantitation, depending on the assay used. As little as 50pg/ml dsDNA can be detected using the standard QuantiFluor® dsDNA Dye protocol. Custom protocols can be created to detect even lower amounts (as little as 1pg/ml dsDNA). The instrument has a linear range that encompasses five orders of magnitude.
Is a computer required to use the Quantus™ Fluorometer?
No. The Quantus™ Fluorometer is designed to operate as a standalone instrument. The Quantus™ Fluorometer displays fluorescence measurements in real time but also saves and displays the 50 most recent measurements in the results history. However, the fluorometer can be connected to a computer to transfer data in real time after installing the free Quantus™ Software. To download the software, visit the Quantus™ Softwaredownload page.
What are the operating system requirements for the optional Quantus™ Software?
To download and use the Quantus™ Software, your computer must have the Windows®10, Windows® 8 or Windows® 7 (32- or 64-bit) operating system, a .NET Framework 4.0 or later and at least one USB port available.
What tubes are compatible with the Quantus™ Fluorometer?
The Quantus™ Fluorometer accepts 0.5ml PCR tubes. We recommend thin-walled PCR tubes (Axygen thin-walled PCR tubes, Axygen Cat.# PCR-05-C, available from Fisher or VWR) for the best optical clarity.
What are the power requirements for the Quantus™ Fluorometer?
The fluorometer requires a power source of 5V, 0.2A maximum. The Quantus™ Fluorometer is supplied with a USB Cord to plug the instrument in to a computer and a USB Power Supply and Outlet Adapter Kit, which includes four adapters, to plug the instrument in to the wall outlet.
How do I calibrate the Quantus™ Fluorometer, and how often should I recalibrate the instrument?
To calibrate, simply measure the fluorescence of your blank sample and the standard that represents the highest point on your standard curve. The Quantus™ Fluorometer saves the calibration data for future measurements. The fluorometer must be calibrated prior to the first use of a protocol. If a protocol that has never been calibrated is selected, the Calibration screen will appear automatically. Once the protocol of interest is calibrated, the Quantus™ Fluorometer will save the calibration values for future use and will not require recalibration. However, we recommend performing a calibration with each new lot of dye reagent to ensure the most accurate measurement.
Does purchase of the Quantus™ Fluorometer include a warranty?
Yes. The Quantus™ Fluorometer comes with a 1-year warranty upon purchase of the instrument. Warranty coverage includes all parts, labor and shipping to and from the repair location.
QuantiFluor is a registered trademark of Promega Corporation. Quantus is a trademark of Promega Corporation. Windows is a registered trademark of Microsoft Corporation.
Protocols
Complete Protocol
Quantus™ Fluorometer Operating ManualPDF (1203 KB) – English
Video Protocols
Specifications
You are viewing: E6150 Change Configuration
SDS
Search for SDS
Certificate of Analysis
Search by lot number
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
Store QuantiFluor® ONE dsDNA Dye and QuantiFluor® ONE Lambda DNA at –30°C to +10°C. Store 1X TE Buffer at –30°C to +30°C.
Specifications
You are viewing: E5150 Change Configuration
What's in the box?
| Item | Part # | Size |
|---|---|---|
Quantus™ Fluorometer | E6150 | 1 × 1 each |
QuantiFluor® ONE dsDNA System | E4870 | 1 × 500 reactions |
0.5ml PCR Tubes | E4941 | 10 × 50 pack |
SDS
Search for SDS
Certificate of Analysis
Search by lot number
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.
Storage Conditions
Store QuantiFluor® ONE dsDNA Dye and QuantiFluor® ONE Lambda DNA at –30°C to +10°C. Store 1X TE Buffer at –30°C to +30°C.
Patents and Disclaimers
U.S. Pat. Nos. 8,598,198 and 9,206,474 and other patents and patents pending.
Resources
Articles
- microRNA Purification from Plant Tissues Using the ReliaPrep™ miRNA Cell and Tissue Miniprep System
- Total RNA Isolation from 3D Cell Cultures or Cells in Matrigel® Matrix Using the ReliaPrep™ miRNA Cell and Tissue Miniprep System
Citations
- Assessment throughout a whole fishing year of the dominant microbiota of peeled brown shrimp (Crangon crangon) stored for 7 days under modified atmosphere packaging at 4°C without preservatives.
--
2016 Food Microbiol. - See all citations
Software and Firmware
- Quantus™ Fluorometer
Other Resources
- Application Notes: Using Quantus™ Fluorometer with Various Quantitation Dyes
- Why Quantitation Matters for NGS
- Quantus™ Fluorometer Sample Measurement
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